Working in the Isotope lab

Type B isotope laboratory (1874-148)

In the type B laboratory, you are allowed to work with larger amounts of isotope than in your local type C laboratories (see tables below).

In order to get access to the new type B laboratory, you will need to have your key-card opened for that room, and it requires an introduction before starting the use of it.

Isotope databases

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Overview of isotope databases at the Department of Molecular Biology and Genetics.

Biokæden" (campus)

When you take an aliquot from an isotope stock, you have to register the amount removed using a key-board instead of pen and paper.

Other locations where stocks of radiochemicals are stored: You can access the isotope database from any computer via the following links:

http://isotopdata.uni.au.dk/BJBisotope/ (for staff members working on the 4^th, 5^th or 6^th floor)

or

http://isotopdata.uni.au.dk/TVSisotope/ (staff members working on the 2^nd or 3^rd floor)

Access-codes are the same as in the hot-room (see below). Fill in all fields, including the volume withdrawn (microliters, integer). When you are done, log out.

If you later realize that you made an error typing the amount withdrawn, contact your purchaser.

The hot-room (1122-016B): You will find a lap-top with just two icons on the desktop: gamma P-32-ATP and alpha P-32 dATP. When you click the relevant icon, you will be prompted for access-codes (see below). When your codes are accepted, you will enter a sheet, where you have to fill in all fields marked with a *. This includes a field, in which you type how many microliters (integer!) you have withdrawn. When your withdrawal is registered, log out. If you forget to log out, your group leader will end up paying for all subsequent withdrawals from the particular vial.

The Science Park:

http://isotope.mb.au.dk/HCTisotope/

MBG iNANO

https://isotopdata.uni.au.dk/isotopeINA/

Isotoplog book (Word-file)

MBG's authorised isotope laboratories

Basic rules for work in the Isotope lab

When handling larger amounts of radioisotopes, please use this lab, but do not leave any of your equipment and stuff down there.
The most recent user(s) will be required to decontaminate, if spillage is detected by a new user or in a routine inspection.
ONLY an authorized isotope purchaser is allowed to purchase radioisotopes, and that purchaser is obliged to maintain proper records of purchase and usage in the Isotope Database.
It is strictly forbidden to “import” radioisotope stock from the Institute’s other department, or any other unauthorized source.
Each group leader is required to teach any relevant staff or student members of his/her group how to handle radioisotopes safely and in accordance with the Institute’s Safety Instructions.

How to become a user

Users

The users are persons with permission to withdraw radiochemicals from stock-vials. If you work with radiochemicals on a regular basis, you should register to become a user. In order to become a user, your purchaser must enter your name into the database.

Useful info: If you already have registered access to the hotroom, you are likely to be registered as “user” in the database. Check the database to see whether it works for you. If not, contact your purchaser for registration.

Quantities of isotope waste must be recorded on lists

According to the rules of isotope work, we must be able to account for the amount of the different isotopes both in stock solutions and in the isotope waste. Therefore, it is important that what is put to waste is also registered on a form. There are printed isotope waste lists in the isotope waste room in the basement for use at the groups' waste bins. The waste must be disposed of after one year. It will therefore be a good idea to switch between waste containers to ensure that activity can come down to 0 after one year.

According to the rules about work with radioactive isotopes, we must to write down the amounts of isotopes both in waste and in stored solutions. It is therefore important that the amount in isotope waste is written on lists.

You can take a new printed list in the isotope waste storage room in the basement to use for your groups isotope waste container.

For P-32 waste the activity will be expected to be 0 when it is sent away from our department. All isotope waste is according to the new rules expected to be sent out from our place after one year. For P-32 waste you have to change between waste containers so that the radioactivity can be 0 after a year.

Please fill in this waste form (Word) - incl. quantity - when disposing of waste.